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rab8 wt  (Addgene inc)
93
Addgene inc rab8 wt
a Summary of membrane turnover and regulation of ciliary length. Ciliary length remains stable because membrane insertion and membrane removal are balanced. Top: Cilia grow by fusion of Golgi-derived vesicles with the periciliary membrane. Blocking vesicle fusion by DN <t>Rab8</t> or brefeldin A blocks growth and reduces ciliary length if membrane removal continues. Elongation of the ciliary membrane is accompanied by elongation of ciliary microtubules (green). Bottom: Endocytosis and ciliary fission reduce ciliary length. Fission can be induced by PIP 2 increase (synthesis or impaired Inpp5e), activation of G-protein coupled receptors (GPCR) or serum exposure. Blocking these processes by PIP 2 depletion, TMSß, DN Rac1, alisertib or tubacin increases ciliary length if vesicle fusion continues. Ciliary microtubules need to be severed for ciliary shortening. b Proposed molecular pathway of ciliary fission. Recruitment of PIPK and deficiency of the 5-phosphatase Inpp5e lead to an increase in ciliary PIP 2 . Serum addition and agonist binding (in the presence of DN dynamin) have the same effect. The PIP 2 increase can be prevented by sequestering PIP 2 using PH(PLCδ1) or by constitutive expression of the 5-phosphatase Inp54p. The PIP 2 increase leads to polymerisation of g-actin to f-actin, presumably amplified by help of the Rho kinases Rac1 and Cdc42. Actin polymerisation can be prevented by TMSß and latruculin A. AurkA and HDAC6 likely act downstream of actin polymerisation and could work by microtubule depolymerisation, which is also required for ciliary fission. AurkA and HDAC are inhibited by alisertib, respectively, tubacin.
Rab8 Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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pcdna3 1 neo  (Addgene inc)
93
Addgene inc pcdna3 1 neo
a Summary of membrane turnover and regulation of ciliary length. Ciliary length remains stable because membrane insertion and membrane removal are balanced. Top: Cilia grow by fusion of Golgi-derived vesicles with the periciliary membrane. Blocking vesicle fusion by DN <t>Rab8</t> or brefeldin A blocks growth and reduces ciliary length if membrane removal continues. Elongation of the ciliary membrane is accompanied by elongation of ciliary microtubules (green). Bottom: Endocytosis and ciliary fission reduce ciliary length. Fission can be induced by PIP 2 increase (synthesis or impaired Inpp5e), activation of G-protein coupled receptors (GPCR) or serum exposure. Blocking these processes by PIP 2 depletion, TMSß, DN Rac1, alisertib or tubacin increases ciliary length if vesicle fusion continues. Ciliary microtubules need to be severed for ciliary shortening. b Proposed molecular pathway of ciliary fission. Recruitment of PIPK and deficiency of the 5-phosphatase Inpp5e lead to an increase in ciliary PIP 2 . Serum addition and agonist binding (in the presence of DN dynamin) have the same effect. The PIP 2 increase can be prevented by sequestering PIP 2 using PH(PLCδ1) or by constitutive expression of the 5-phosphatase Inp54p. The PIP 2 increase leads to polymerisation of g-actin to f-actin, presumably amplified by help of the Rho kinases Rac1 and Cdc42. Actin polymerisation can be prevented by TMSß and latruculin A. AurkA and HDAC6 likely act downstream of actin polymerisation and could work by microtubule depolymerisation, which is also required for ciliary fission. AurkA and HDAC are inhibited by alisertib, respectively, tubacin.
Pcdna3 1 Neo, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna3 1 neo/product/Addgene inc
Average 93 stars, based on 1 article reviews
pcdna3 1 neo - by Bioz Stars, 2026-02
93/100 stars
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flag rab8a  (Addgene inc)
93
Addgene inc flag rab8a
a Summary of membrane turnover and regulation of ciliary length. Ciliary length remains stable because membrane insertion and membrane removal are balanced. Top: Cilia grow by fusion of Golgi-derived vesicles with the periciliary membrane. Blocking vesicle fusion by DN <t>Rab8</t> or brefeldin A blocks growth and reduces ciliary length if membrane removal continues. Elongation of the ciliary membrane is accompanied by elongation of ciliary microtubules (green). Bottom: Endocytosis and ciliary fission reduce ciliary length. Fission can be induced by PIP 2 increase (synthesis or impaired Inpp5e), activation of G-protein coupled receptors (GPCR) or serum exposure. Blocking these processes by PIP 2 depletion, TMSß, DN Rac1, alisertib or tubacin increases ciliary length if vesicle fusion continues. Ciliary microtubules need to be severed for ciliary shortening. b Proposed molecular pathway of ciliary fission. Recruitment of PIPK and deficiency of the 5-phosphatase Inpp5e lead to an increase in ciliary PIP 2 . Serum addition and agonist binding (in the presence of DN dynamin) have the same effect. The PIP 2 increase can be prevented by sequestering PIP 2 using PH(PLCδ1) or by constitutive expression of the 5-phosphatase Inp54p. The PIP 2 increase leads to polymerisation of g-actin to f-actin, presumably amplified by help of the Rho kinases Rac1 and Cdc42. Actin polymerisation can be prevented by TMSß and latruculin A. AurkA and HDAC6 likely act downstream of actin polymerisation and could work by microtubule depolymerisation, which is also required for ciliary fission. AurkA and HDAC are inhibited by alisertib, respectively, tubacin.
Flag Rab8a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/flag rab8a/product/Addgene inc
Average 93 stars, based on 1 article reviews
flag rab8a - by Bioz Stars, 2026-02
93/100 stars
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pcdna3 1neo  (Addgene inc)
93
Addgene inc pcdna3 1neo
a Summary of membrane turnover and regulation of ciliary length. Ciliary length remains stable because membrane insertion and membrane removal are balanced. Top: Cilia grow by fusion of Golgi-derived vesicles with the periciliary membrane. Blocking vesicle fusion by DN <t>Rab8</t> or brefeldin A blocks growth and reduces ciliary length if membrane removal continues. Elongation of the ciliary membrane is accompanied by elongation of ciliary microtubules (green). Bottom: Endocytosis and ciliary fission reduce ciliary length. Fission can be induced by PIP 2 increase (synthesis or impaired Inpp5e), activation of G-protein coupled receptors (GPCR) or serum exposure. Blocking these processes by PIP 2 depletion, TMSß, DN Rac1, alisertib or tubacin increases ciliary length if vesicle fusion continues. Ciliary microtubules need to be severed for ciliary shortening. b Proposed molecular pathway of ciliary fission. Recruitment of PIPK and deficiency of the 5-phosphatase Inpp5e lead to an increase in ciliary PIP 2 . Serum addition and agonist binding (in the presence of DN dynamin) have the same effect. The PIP 2 increase can be prevented by sequestering PIP 2 using PH(PLCδ1) or by constitutive expression of the 5-phosphatase Inp54p. The PIP 2 increase leads to polymerisation of g-actin to f-actin, presumably amplified by help of the Rho kinases Rac1 and Cdc42. Actin polymerisation can be prevented by TMSß and latruculin A. AurkA and HDAC6 likely act downstream of actin polymerisation and could work by microtubule depolymerisation, which is also required for ciliary fission. AurkA and HDAC are inhibited by alisertib, respectively, tubacin.
Pcdna3 1neo, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna3 1neo/product/Addgene inc
Average 93 stars, based on 1 article reviews
pcdna3 1neo - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier

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a Summary of membrane turnover and regulation of ciliary length. Ciliary length remains stable because membrane insertion and membrane removal are balanced. Top: Cilia grow by fusion of Golgi-derived vesicles with the periciliary membrane. Blocking vesicle fusion by DN Rab8 or brefeldin A blocks growth and reduces ciliary length if membrane removal continues. Elongation of the ciliary membrane is accompanied by elongation of ciliary microtubules (green). Bottom: Endocytosis and ciliary fission reduce ciliary length. Fission can be induced by PIP 2 increase (synthesis or impaired Inpp5e), activation of G-protein coupled receptors (GPCR) or serum exposure. Blocking these processes by PIP 2 depletion, TMSß, DN Rac1, alisertib or tubacin increases ciliary length if vesicle fusion continues. Ciliary microtubules need to be severed for ciliary shortening. b Proposed molecular pathway of ciliary fission. Recruitment of PIPK and deficiency of the 5-phosphatase Inpp5e lead to an increase in ciliary PIP 2 . Serum addition and agonist binding (in the presence of DN dynamin) have the same effect. The PIP 2 increase can be prevented by sequestering PIP 2 using PH(PLCδ1) or by constitutive expression of the 5-phosphatase Inp54p. The PIP 2 increase leads to polymerisation of g-actin to f-actin, presumably amplified by help of the Rho kinases Rac1 and Cdc42. Actin polymerisation can be prevented by TMSß and latruculin A. AurkA and HDAC6 likely act downstream of actin polymerisation and could work by microtubule depolymerisation, which is also required for ciliary fission. AurkA and HDAC are inhibited by alisertib, respectively, tubacin.

Journal: Communications Biology

Article Title: PIP 2 determines length and stability of primary cilia by balancing membrane turnovers

doi: 10.1038/s42003-022-03028-1

Figure Lengend Snippet: a Summary of membrane turnover and regulation of ciliary length. Ciliary length remains stable because membrane insertion and membrane removal are balanced. Top: Cilia grow by fusion of Golgi-derived vesicles with the periciliary membrane. Blocking vesicle fusion by DN Rab8 or brefeldin A blocks growth and reduces ciliary length if membrane removal continues. Elongation of the ciliary membrane is accompanied by elongation of ciliary microtubules (green). Bottom: Endocytosis and ciliary fission reduce ciliary length. Fission can be induced by PIP 2 increase (synthesis or impaired Inpp5e), activation of G-protein coupled receptors (GPCR) or serum exposure. Blocking these processes by PIP 2 depletion, TMSß, DN Rac1, alisertib or tubacin increases ciliary length if vesicle fusion continues. Ciliary microtubules need to be severed for ciliary shortening. b Proposed molecular pathway of ciliary fission. Recruitment of PIPK and deficiency of the 5-phosphatase Inpp5e lead to an increase in ciliary PIP 2 . Serum addition and agonist binding (in the presence of DN dynamin) have the same effect. The PIP 2 increase can be prevented by sequestering PIP 2 using PH(PLCδ1) or by constitutive expression of the 5-phosphatase Inp54p. The PIP 2 increase leads to polymerisation of g-actin to f-actin, presumably amplified by help of the Rho kinases Rac1 and Cdc42. Actin polymerisation can be prevented by TMSß and latruculin A. AurkA and HDAC6 likely act downstream of actin polymerisation and could work by microtubule depolymerisation, which is also required for ciliary fission. AurkA and HDAC are inhibited by alisertib, respectively, tubacin.

Article Snippet: Rab8 WT in pcDNA3.1neo was used as control and a gift from Terry Hébert as well (Addgene plasmid # 46783).

Techniques: Membrane, Derivative Assay, Blocking Assay, Activation Assay, Binding Assay, Expressing, Amplification